Open Access Open Access  Restricted Access Subscription or Fee Access

Bioinformatical Analysis of HGPRT Transferase from Different Malaria Parasite Plasmodium spp. Using Computational Tools

Nahla Osman Mohamed Ali

Abstract


In this study, HGPRT transferases from different malaria parasite Plasmodium species was analyzed and presented in this communication. The composition of leucine, lysine and Isoleucine were the highest while lowest concentrations of tryptophan and glutamine residues were noticed when compared to other amino acids. pI value of P. reichenowi HGPRT was 7.59 while the lowest pI of 6.22 was shown by P. chabaudi HGPRT. The instability index of all the transferases is varied, but for all of them it was less than 40, which indicates that all of them are stable. The aliphatic index was found to span within a range of 83 to 97. Secondary structural analysis of the transferases showed the pre-dominance of random coils followed by extended strands for all the transferases except P. falciparum, P. Knowlesi and P. reichenowi HGPRT transferase. The significance of the above results is discussed in the light of existing literature.

 

Handle: http://hdl.handle.net/20.500.11903/mjmbr.v2n3.12

 


Keywords


HGPRT – Phosphoribosyltransferase - Plasmodium spp. - Bioinformatics – Secondary structure

Full Text:

PDF

References


Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215: 403-10.

Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-402.

Bahl A, Brunk B, Coppel RL, Crabtree J, Diskin SJ, Fraunholz MJ, Grant GR, Gupta D, Huestis RL, Kissinger JC, Labo P, Li L, McWeeney SK, Milgram AJ, Roos DS, Schug J, Stoeckert CJ Jr. (2002) PlasmoDB: the Plasmodium genome resource. An integrated database providing tools for accessing, analyzing and mapping expression and sequence data (both finished and unfinished). Nucleic Acids Res. 2002 Jan 1;30(1):87-90.

Bairoch A. and R. Apweiler R. (2000). “The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000,†Nucl. Acids Res., vol. 28, pp. 45–48, 2000.

Berman PA, Human L, Freese JA. (1991) Xanthine oxidase inhibits growth of Plasmodium falciparum in human erythrocytes in vitro. J Clin Invest. 1991 Dec;88(6):1848-55.

Combet C., Blanchet C., Geourjon C. and Deléage G. (2000). NPS@: Network Protein Sequence Analysis. TIBS 2000 March Vol. 25, No 3 [291]:147-150.

Craig SP 3rd and Eakin AE. (2000) Purine phosphoribosyltransferases. J Biol Chem. 2000 Jul 7;275(27):20231-4.

Eads JC, Ozturk D, Wexler TB, Grubmeyer C, Sacchettini JC. (1997) A new function for a common fold: the crystal structure of quinolinic acid phosphoribosyltransferase. Structure. 1997 Jan 15;5(1):47-58.

Gasteiger E., Jung E. Bairoch A. (2001). “SWISS-PROT: Connecting biological knowledge via a protein database,†Curr. Issues Mol. Biol., vol. 3, pp. 47–55, 2001.

Geourjon C. and Deleage G. (1995). “SOPMA: Significant Improvements in protein secondary structure prediction by consensus prediction from multiple alignments,†Comput. Appl. Biosci., vol. 11, pp. 681–684, 1995.

Gomi M., Sonoyama M., and Mitaku S. (2004). High performance system for signal peptide prediction: SOSUIsignal Chem-Bio Info. J., 4 :142-147.

HersheyH.V.,TaylorM.W.NucleotidesequenceanddeducedaminoacidsequenceofEscherichiacoliadeninephosphoribosyltransferase and comparison with other analogous enzymes. Gene 43:287-293(1986).

Musick WD. (1981) Structural features of the phosphoribosyltransferases and their relationship to the human deficiency disorders of purine and pyrimidine metabolism. CRC Crit Rev Biochem. 1981;11(1):1-34.

Olliaro PL & Yuthavong Y (1999) An overview of chemotherapeutic targets for antimalarial drug discovery. Pharmacol Ther 81: 91–110.

Pagni M. Ioannidis V. Cerutti L. Zahn-Zabal M. Jongeneel C. Jo¨rg H. Olivier M. Dmitri K. Laurent F. (2007) “MyHits: Improvements to an interactive resource for analyzing protein sequences,†Nucleic Acids Res., vol. 35, pp. W433–W437, 2007.

Queen SA, Vander Jagt DL, Reyes P. (1988) Properties and substrate specificity of a purine phosphoribosyltransferase from the human malaria parasite, Plasmodium falciparum. Mol Biochem Parasitol. 1988 Aug;30(2):123-33.

Reyes P, Rathod PK, Sanchez DJ, Mrema JE, Rieckmann KH, Heidrich HG. (1982) Enzymes of purine and pyrimidine metabolism from the human malaria parasite, Plasmodium falciparum. Mol Biochem Parasitol. 1982 May;5(5):275-90.

Shahabuddin M, Scaife J. (1990) The gene for hypoxanthine phosphoribosyl transferase of Plasmodium falciparum complements a bacterial HPT mutation. Mol Biochem Parasitol. 1990 Jun;41(2):281-8.

Sharma V, Grubmeyer C, Sacchettini JC. (1998) Crystal structure of quinolinic acid phosphoribosyltransferase from Mycobacterium tuberculosis: a potential TB drug target. Structure. 1998 Dec 15;6(12):1587-99.

Sherman I. W. (1979) Biochemistry of Plasmodium (malarial parasites). Microbiol Rev. 1979 Dec;43(4):453-95.

Sujay Subbayya IN and Balaram H (2000). Evidence for multiple active states of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase. Biochem Biophys Res Commun 279: 433–437.

Ullman, B., Carter, D. (1995) Hypoxanthine-guanine phosphoribosyltransferase as a therapeutic target in protozoal infections. Infect Agents Dis. 1995 Mar;4(1):29-40.

Vasanthakumar G, Davis RL Jr, Sullivan MA, Donahue JP. (1990) Cloning and expression in Escherichia coli of a hypoxanthine-guanine phosphoribosyltransferase-encoding cDNA from Plasmodium falciparum. Gene. 1990 Jul 2;91(1):63-9.

Webster HK, Wiesmann WP, Pavia CS. (1984) Adenosine deaminase in malaria infection: effect of 2’-deoxycoformycin in vivo. Adv Exp Med Biol. 1984;165 Pt A:225-9.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Nahla Osman Mohamed Ali

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.