Evaluation of Antibacterial Property of Cell-free Hemolymph and Hemocytes of Marine Gastropod, Rapana Rapiformis from Inshore Waters of Pondicherry, Southeast Coast of India
DOI:
https://doi.org/10.18034/mjmbr.v4i1.423Keywords:
Marine gastropod, Rapana rapiformis, antibacterial, vibrio cholera, low molecular peptideAbstract
The cell –free hemolymph and hemocytes isolated from body-fluid of marine gastropod Rapana rapiformis distributed in the shallow waters of Pondichery coast, were tested for their antibiotic potential against five common human bacterial pathogens viz. Klebsiella pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio cholera and E.Coli. The sensitivity test thro Disc diffusion assay revealed that cell–free hemolymph of Rapana rapiformis exhibited higher level of inhibition even at MIC of 2.5ul against Vibrio cholera when compared to reference drug. A peptide molecule in haemolymph that showed higher inhibitory activity with the molecular mass of 35kDa, has been found by Sodium Dodecyl Sulphate Polyacrylamide gel electrophoresis. It could be of greater interest to isolate and characterize this protein which might be used commercially against existing antibiotic resistant strains such as MRSA in future.
Metrics
Downloads
References
Anitha and K. Sharath, Antimicrobial peptides from the haemolymph of crabs Scylla serrata and Metapograpsus messor. Asiatic Journal of Biotechnology Resources; 2011, 2 (5) 568-576.
Armstrong A D, Armstrong J L, Krassner S M and Pauley G B.,. Experimental wound repair in the black abalone, Holiotis cracherodii. Journal of Invertebrate Pathology, 1971, 17: 216-227 DOI: https://doi.org/10.1016/0022-2011(71)90094-2
Chen J H and Bayne C J., Bivalve mollusk haemocyte behaviors: Charecterisation of hemocytes aggregation and adhesion and their inhibition in the california mussel (mytilus californianus).Biological bulletin, 1995, 188:255-266. DOI: https://doi.org/10.2307/1542303
Cheng, T. C., A classification of molluscan hemocytes based on functional evidences. In Invertebrate Blood, 1984, 111-146. DOI: https://doi.org/10.1007/978-1-4684-4766-8_5
Dunphy, G.B., U. Oberholzer, M. Whiteway, R.J. Zakarian and I. Boomer. Virulence of Candida albicans mutants toward larvae of Galleria mellonella (Insecta, Lepidoptera, Galleridae). Can J Microbiol, 2003, 49:514-524. DOI: https://doi.org/10.1139/w03-064
Irving, P., L. Troxler and C. Hetru, Is innate enough? The innate immune response in Drosophila. CR Biol.2004, 327: 557-570. DOI: https://doi.org/10.1016/j.crvi.2004.03.007
Jarosz, J., G. Kania & M. Balcerzak,Cell-free immune entities of four diplopod myriapods. – In: Program and abstracts, XXIVAnnual Meeting, Society for Invertebrate Pathology, Flagstaff, Arizona, 4.–9.8.1991: 100.
Juliet, M.Relf, June R.S.Chisholm, Graham D.Kemp and Valerie J.Smith, Purififaction and characterization of a cysteine-rich 11.5kDa antibacterial protein from granula hemocytes of the shore crab, Crcinus maenas. Eur.J.Biochem.1999, 267:677-683.
Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227(5259), 680-685. McCaffrey, E. J., & Endean, R.Antimicrobial activity of tropical and subtropical sponges. Marine Biology, 1985, 89(1), 1-8.
Miyata, T., Tokunaga, F., Yoneya, T., Yoshikawa, K., Iwanaga, S., Niwa, M., & Shimonishi, Y.Antimicrobial peptides, isolated from horseshoe crab hemocytes, tachyplesin II, and polyphemusins I and II: chemical structures and biological activity. Journal of biochemistry, 1989, 106(4), 663-668. DOI: https://doi.org/10.1093/oxfordjournals.jbchem.a122913
Mount A S, Wheeler A P, Paradkar R P and Snider D. Hemocytes-mediated shell mineralisation in the eastern oyster. Science, 2004,304, 297-300. DOI: https://doi.org/10.1126/science.1090506
Nevermann, L. & W. E. R. Xylander,In vitro cellular immune reactions of hemocytes against bacteria and their differential degradation in myriapods. – In: Geoffroy, J.-J., Mauries & M. Nguyen Duy-Jacquemin (eds): Acta Myriapodologica Mémoires du Muséum national d`Histoire naturelle, 199, 169, 421– 430.
Nevermann, L., H. E. Kaiser & W. E. R Xylander,Microbial induced haemocytic immune reactions in chilopods. – In Vivo, 1996,10, 161–168.
Ottavini E, Caselgrandi, Petraglia F and Francheschi C, Stress responses in the freshwater snail plauorbarius corneus (l.) (gastropoda, plumonata):interaction between CRF, ACTH and biogenic amines. General and comparitive endocrinology 1996, 87(3), 354-360. DOI: https://doi.org/10.1016/0016-6480(92)90041-H
Ottavini E, Franchini A, Fontanili P,The presence of immunoreactive vertebrate bioactive peptide substances in hemocytes of the freshwater snail viviparus ater (gastropoda, prosobranchia). Cellular and molecular neurobiology, 1992. 12,455-462. DOI: https://doi.org/10.1007/BF00711546
Pan, J. Y., Zhang, Y. L., Wang, S. Y., & Peng, X. X. Dodecamer is required for agglutination of Litopenaeus vannamei hemocyanin with bacterial cells and red blood cells. Marine Biotechnology, 2008, 10(6), 645-652. DOI: https://doi.org/10.1007/s10126-008-9115-8
Pitchiah Sivaperumal, Kannan Kamala, Elayaperumal Natarajan, Elangovan Dilipan., Antimicrobial peptide from crab haemolymph of Ocypoda macrocera (Milne Edwards 1852) with reference to antioxidant: a case study. Int J Pharm Pharm Sci, 2013, 5, 719-727.
Pollero R J, Huca G and Brenuer, Role of hemocytes and plasma on lipid transport in fresh water mollusc Diplodon delodontus. Comparitive biochemistry and physiology, 1985, 82,339-343. DOI: https://doi.org/10.1016/0300-9629(85)90865-5
Rajaganapathy, J., Kathiresan, K and Sing, T.P, Purification Anti-HIV protein from purple fluid of the seahare Bursatella leachii de Blainville. J. Mar. Biotechnol, 2000, 4,447-453. DOI: https://doi.org/10.1007/s10126-002-0012-2
Rameshkumar, G., Ravichandran, S., Kaliyavarathan, G., & Ajithkumar, T. T. Antimicrobial peptide from the crab, Thalamita crenata (Latreille, 1829). World J Fish Mar Sci, 2009, 1(2), 74-79.
Rameshkumar, G., S. Ravichandran and T. Aravindhan, Antimicrobial Proteins from the Crab Charybdis lucifera (Fabricius, 1798). Middle East J. Sci. Res, 2009, 4, 40-43.
Rinehart, K.L., P.D. Shaw, L.S. Shield, J.B. Gloer, G.C. Harbour and M.E.S. Koker, Marine naturaL products as sources of antiviral, antimicrobial and antineoplastic agents. Pure and Applied Chemistry, 1981, 53, 795-817. DOI: https://doi.org/10.1351/pac198153040795
Saito, T.S. Kawabata, M.Hirata and S.Iwanaga, A novel type of Limulus Lectin-6: purification, primary structure and antibacterial activity. The American Society for Biochemistry and Molecular Biology, 1995, 270, 14493-14499. DOI: https://doi.org/10.1074/jbc.270.24.14493
Samuthirapandian Ravichandran, Singaram Jeyalakshmi, Sathasivam Sudha and Ramasamy Anbuchezhian, Antimicrobial peptides from the haemolymph of the prawn Macrobrachium rosenbergii. Bangladesh J. Pharmacol.2010, 5, 62-67.
Seraj, U. M., Hoq, M. I., Anwar, M. N., & Chowdhury, A 61kDa Antibacterial Protein Isolated and Purified from the Hemolymph of the American Cockroach Periplaneta amreicana. Pakistan Journal of Biological Sciences, 2003, 6(7), 715-720. DOI: https://doi.org/10.3923/pjbs.2003.715.720
Sharmin Vini, S., Asha, A., Viju N., Sunjaiy Shankar, C. V. and Mary Josephine Punitha.S, Antibacterial activity of hemolymph of selected gastropods sps collected from kanyakumari coast against human pathogens. International Journal of Current Research, 2013, 5(12), 4066-4069.
Thormar, H., & Hilmarsson, H. The role of microbicidal lipids in host defense against pathogens and their potential as therapeutic agents. Chemistry and physics of lipids, 2007, 150(1), 1-11. DOI: https://doi.org/10.1016/j.chemphyslip.2007.06.220
Tzou, P., E. De Gregorio and B. Lemaitre, (2002). How Drosophila combats microbial infections: a model to study innate immunity and host –pathogen interactions. Curr Opin Microbiol.2002, 5, 102-110. DOI: https://doi.org/10.1016/S1369-5274(02)00294-1
Van der Walt, E., L. McClain, A. Puren & N. Savage,Phylogeny of arthropod immunity. An inducible humoral response in the Kalahari millipede, Triaenostreptus triodus (Attems). – Naturwissenschaften, 1990, 77, 89–90. DOI: https://doi.org/10.1007/BF01131166
Xylander, W. E. Epidermis and sensory receptors of Temnocephala minor (Plathelminthes, Rhabdocoela, Temnocephalida): an electron microscopic study. Zoomorphology, 1997, 117(3), 147-154. DOI: https://doi.org/10.1007/s004350050039
Xylander, W. E. Physico-chemical properties of haemolymph of Chilopoda and Diplopoda (Myriapoda, Arthropoda): protein content, pH, osmolarity. Soil Org, 2009, 81, 431-439.
Xylander, W. E. R. & L. Nevermann, Antibacterial activity in the hemolymph of Myriapoda (Arthropoda). – Journal of Invertebrate Pathology, 1990, 56(2), 206–214 DOI: https://doi.org/10.1016/0022-2011(90)90102-C
Xylander, W. E. R. Antibacterial substances from the hemolymph of various myriapods. –Symposium on Invertebrate Immunology, Lecce/Italy, 1989, 25. Abstracts: 33.
Xylander, W. E. R. Immune defense reactions of Myriapoda – A brief presentation of recent results. In: Thaler, K., E. Meyer & W. Schedl (eds): Advances in Myriapodology (Proceedings of the 8th International Congress of Myriapodology). – Berichte des naturwissenschaftlich-medizinischen Vereines Innsbruck, Suppl.1992, 10, 101–110.
-- 0 --
