Regenerative Effects of Wharton’s Jelly Stem Cells-Conditioned Medium in UVA-Irradiated Human Dermal Fibroblasts
DOI:
https://doi.org/10.18034/mjmbr.v5i1.449Keywords:
collagen depositions, migration, photo-ageing fibroblasts, proliferations, TGF-β, UVA, Wharton’s jelly stem cells conditioned-mediumAbstract
Background: Ultraviolet A radiation (UVA) can photo-age skin by suppressing the proliferation, migration, and collagen deposition of human dermal fibroblasts (HDFs). This process occurs because UVA light can inhibit the gene expression of the TGF-β receptor in HDFs. Moreover, Wharton’s Jelly Stem Cells-Conditioned Medium (WJSC-CM) is hypothesized to release microvesicles that contain short m-RNA with regenerative properties.
Objectives: This study aimed to determine the regenerative properties of WJSC-CM on UVA-Irradiated Human Dermal Fibroblasts (UVA-HDFs)
Methods: Passaged fourth of HDFs obtained from the foreskin of six (11- to 13-year-old) boys were repeatedly irradiated with a total of 10 J/cm2 UVA and treated with various concentrations of WJSC-CM. We used non-irradiated HDFs as positive control. After that, the consumption of TGF-β, cellular proliferation, cellular migration, and collagen deposition of each group were measured and compared.
Results: Compared to the non-irradiated groups, the proliferation rates, migration rates, and collagen deposition of UVA-HDFs significantly decreased (p<0.05). WJSC-CM can improve the consumption of TGF-β, proliferation, and cellular migration of UVA-HDFs. However, WJSC-CM failed to improve the collagen deposition of UVA-HDFs (p>0.05).
Conclusions: WJSC-CM has regenerative properties and is a candidate material for the treatment of prematurely ageing skin induced by UVA-irradiation.
Ethical permission: This experiment was permitted by the local ethical permission committee Ref:KE/FK/382/EC with permission letter dated 17-04-2013.
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